The mission of the Georgia Cancer Center's Immune Monitoring Shared Resource (IMSR) laboratory is to provide comprehensive immune monitoring services and expertise to assist researchers and clinicians in designing and performing basic and translational research projects.
The main objective of the resource is to provide standardized immune-based assays, to develop, optimize, and validate new technologies, and to support efforts in the areas of human and animal immunology and immunotherapy.
For more information please go to the Immune Monitoring section on the Shared Resources page.
Immune Monitoring
Health Sciences Campus
GCC - M. Bert Storey Research Building
Valentyna Fesenkova, PhD
IMSR Research Manager
CN-4135A
(706) 446-5512
Rafal Pacholczyk, PhD
Director
CN-4132
(706) 721-3528
Immune Cell Phenotyping by Flow Cytometry
Multi-color flow cytometric analysis allows characterization of the immune phenotypes (e.g., immune cell subsets, activation & differentiation status) of different immune cell populations in human and animal models. We offer custom designed panels based on individual customer needs.
Intracellular staining of transcription factors (e.g., Foxp3) and cytokines to determine simultaneously the phenotype of cells and their cytokine production profile. We also offer several options for in vitro stimulation (e.g., LPS, PMA+Ionomycin, anti-CD3 and CD28 stimulation) of PBMCs for cytokine profiling.
Luminex
The Luminex technology allows for the measurement of up to 100 distinct proteins through a suspension bead array that employs a series of distinct color-coded microspheres to simultaneously detect multiple soluble analytes, from a single serum, plasma, tissue culture supernatant, or other bodily fluid sample. (Kits available from Bio-Rad, Thermo, R&D, Millipore etc)
ELISA
Detection of a single analyte using commercially available standard or competitive ELISA assays.
Next-Gen TCR Sequencing
We offer project based high - throughput sequencing of human T cell receptors (TCRs) from RNA or DNA.
From RNA: simultaneous preparation of barcoded TCRalpha and TCRbeta library (PCR-unbiased, using template-switching RT PCR technology) for Illumina Mi-SEQ PE sequencing
From genomic DNA: preparation of barcoded TCRbeta - targeted library for Illumina Mi-SEQ PE sequencing
Service includes library pooling, MI-SEQ sequencing in collaboration with our Integrated Genomics Resource, QC quantitation, CDR3 clone extraction and annotation (alignment, error correction, V (D) J and CDR3 assignment). We also offer high level TCR analysis including diversity measure, frequency distribution, clone tracking etc.
Please, inquire for mouse TCR repertoire sequencing.
Flow-Based Bead Array
Measurement of soluble proteins (up to 13 per sample) in plasma/serum, supernatants and selected cell lysates using flow cytometry. This technology utilizes the same basic principles of sandwich immunoassays, whereby a soluble analyte is captured between two antibodies. (Kits available from Biolegend or BD Biosciences)
Immune Celle Isolation
PBMC isolation
Magnetic-bead based isolation of immune cell subsets (e.g., T cells, B cells and monocytes) from whole blood samples or PBMCs.
Please, inquire about non-human cell isolation
Consultation/Data Analysis
Consultation - Experimental design discussion
Data Analysis - Custom data processing and analysis
Grant/Manuscript Assistance - Assistance in writing sections of your manuscript or grant applications involving services provided by the IMSR
