Immune Monitoring

Immune Cell Phenotyping by Flow Cytometry

Multi-color flow cytometric analysis allows characterization of the immune phenotypes (e.g., immune cell subsets, activation & differentiation status) of different immune cell populations in human and animal models. We offer custom designed panels based on individual customer needs.

Intracellular staining of transcription factors (e.g., Foxp3) and cytokines to determine simultaneously the phenotype of cells and their cytokine production profile. We also offer several options for in vitro stimulation (e.g., LPS, PMA+Ionomycin, anti-CD3 and CD28 stimulation) of PBMCs for cytokine profiling.

 

Luminex

The Luminex technology allows for the measurement of up to 100 distinct proteins through a suspension bead array that employs a series of distinct color-coded microspheres to simultaneously detect multiple soluble analytes, from a single serum, plasma, tissue culture supernatant, or other bodily fluid sample. (Kits available from Bio-Rad, Thermo, R&D, Millipore etc)

ELISA

Detection of a single analyte using commercially available standard or competitive ELISA assays.

 

Next-Gen TCR Sequencing

We offer project based high - throughput sequencing of human T cell receptors (TCRs) from RNA or DNA.

From RNA:  simultaneous preparation of barcoded TCRalpha and TCRbeta library (PCR-unbiased, using template-switching RT PCR technology) for Illumina Mi-SEQ PE sequencing

From genomic DNA: preparation of barcoded TCRbeta - targeted library for Illumina Mi-SEQ PE sequencing

Service includes library pooling, MI-SEQ sequencing in collaboration with our Integrated Genomics Resource, QC quantitation, CDR3 clone extraction and annotation (alignment, error correction, V (D) J and CDR3 assignment). We also offer high level TCR analysis including diversity measure, frequency distribution, clone tracking etc.

Please, inquire for mouse TCR repertoire sequencing.

Flow-Based Bead Array

Measurement of soluble proteins (up to 13 per sample) in plasma/serum, supernatants and selected cell lysates using flow cytometry. This technology utilizes the same basic principles of sandwich immunoassays, whereby a soluble analyte is captured between two antibodies.  (Kits available from Biolegend or BD Biosciences)

 

Immune Celle Isolation

PBMC isolation

Magnetic-bead based isolation of immune cell subsets (e.g., T cells, B cells and monocytes) from whole blood samples or PBMCs.

Please, inquire about non-human cell isolation

 

Consultation/Data Analysis

Consultation - Experimental design discussion

Data Analysis - Custom data processing and analysis

Grant/Manuscript Assistance - Assistance in writing sections of your manuscript or grant applications involving services provided by the IMSR