Research Interest, Education & Publications
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The Lambert Lab studies the molecular pharmacology of G protein-coupled receptors (GPCRs) and their downstream signaling partners. We use cell-based and biophysical approaches to ask questions about how GPCRs, G proteins and effectors are arranged and interact in cells.
1990 BS, Youngstown State University
1991 PhD, Kent State University
1996 Postdoctoral, Duke University
Regents’ Professor
Vice-Chair, Department of Pharmacology and Toxicology
Journal of Biological Chemistry
Molecular Pharmacology
Frontiers in Endocrinology
Molecular Neuropharmacology and Signaling (MNPS), NIH (chair)
My dog seems to like me
Jang, W., Lu, S., Xu, X., Wu, G. and Lambert, N.A. The role of G protein conformation in receptor-G protein selectivity. Nature Chemical Biology, 19:687–694, 2023.
Asher, W.B., Terry, D.S., Gregorio, G.G.A., Kahsai, A.W., Borgia, A., Xie, B., Modak, A., Zhu, Y., Jang, W., Govindaraju, A., Huang, L., Inoue, A., Lambert, N.A., Gurevich, V.V., Shi, L., Lefkowitz, R.J., Blanchard, S.C. and Javitch, J.A. GPCR-mediated b-arrestin Activation Deconvoluted with Single-molecule Precision. Cell, 185(10):1661-1675, 2022.
Wright, S.C., Lukasheva, V., Le Gouill, C., Kobayashi, H., Breton, B., Mailhot-Larouche, S., Blondel-Tepaz, É, Vieira, N.A., Costa-Neto, C., Héroux, M., Lambert, N.A., Parreiras-e-Silva, L.T. and Bouvier, M. BRET-based effector membrane translocation assay monitors GPCR-promoted and endocytosis-mediated Gq activation at early endosomes. Proc. Natl. Acad. Sci. USA, 118(20):e2025846118, 2021.
Lu, S., Jang, W., Inoue, A. and Lambert N.A. Constitutive G protein coupling profiles of understudied orphan GPCRs. PLOS ONE, 16(4):e0247743, 2021.
Jang, W., Adams, C.E., Liu, H., Zhang, C., Levy, F.O., Andressen, K.W. and Lambert, N.A. An inactive receptor-G protein complex maintains the dynamic range of agonist-induced signaling. Proc. Natl. Acad. Sci. USA, 117 (48):30755-30762, 2020.
Okashah, N., Wright, S.C., Kawakami, K., Mathiasen, S., Zhou, J., Lu, S., Javitch, J.A., Inoue, A., Bouvier, M. and Lambert, N.A. Agonist-induced formation of unproductive receptor-G12 complexes. Proc. Natl. Acad. Sci. USA, 117(35):21723-21730, 2020.
Bondar, A., Jang, W., Sviridova, E. and Lambert, N.A. Components of the Gs signaling cascade exhibit distinct changes in mobility and membrane domain localization upon 2-adrenergic receptor activation. Traffic, 21(4):324-332, 2020.
Okashah, N. Wan, Q., Ghosh, S., Sandhu, M., Inoue, A., Vaidehi, N. and Lambert, N.A. Variable G protein determinants of GPCR coupling selectivity. Proc. Natl. Acad. Sci. USA, 116 (24):12054-12059, 2019.
Wan, Q., Okashah, N., Inoue, A., Nehmé, R., Carpenter, B., Tate, C.G. and Lambert, N.A. Mini G protein probes for active G protein-coupled receptors (GPCRs) in live cells. Journal of Biological Chemistry, 293(19) 7466 –7473, 2018. (selected as “Editor’s Pick”; most read JBC paper of 2018)
