The Integrated Genomics Shared Resource in the Georgia Cancer Center offer cutting edge technologies for DNA and RNA sequencing as well as spatial transcriptomic.​

We offer a wide range of services from sample processing from both fresh and frozen, as well as fixed cells or tissues to library preparation and next-generation sequencing (NGS) for bulk DNA/RNA sequencing and single-cell/nuclei sequencing. For single-cell studies, we are equipped with the Chromium X system from 10X Genomics. Our sequencing capabilities include the Illumina Novaseq6000 and NextSeq 500. We recently introduce single cell spatial transcriptomics with CosMx SMI platform been able to provide support to investigator that are focusing on single cell in tissue in a specific microenvironment. We are also equipped with CytAssit instrument from 10X genomics for VISIUM spatial transcriptomic. For nucleic acid and protein extraction we are equipped with QIAConnect from Qiagen.

For more information please see the Integrated Genomics Additional Info on the Shared Resources page.

 

Equipment & Services

Users are responsible for knowing how to run the equipment and what products and sample preparation to use.

All services are booked through iLAB

Contact Us

Integrated Genomics

CN 3162

Martina Zoccheddu
​, PhD -
Director

Miao Yu, PhD
Research Manager

(706) 721-5471

mzoccheddu@augusta.edu

miyu@augusta.edu

StepOnePlus™ Real-Time PCR

StepOnePlus™ Real-Time PCR

The StepOnePlus Real-Time PCR instrument utilizes an easy-to-use interface and program wizards to help guide the researchers through the process of setting up a run.

It runs on a 96-well format, and uses up to 4 colored dyes. The instrument can be used for multiple applications, including:

  • Gene Expression Profiling
  • SNP Genotyping, MicroRNA Expression
  • Translocation Analysis, Viral Load AnalysisGene Detection

COST

The service fee is $10 per use for runs on the StepOnePlus Real-Time PCR instrument.

Qiagen instrument
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Qiagen |Sample Purification

QIAcube Connect is designed to perform fully automated purification of nucleic acids and proteins in molecular biology applications. The system is intended for use by professional users trained in molecular biological techniques and the operation of QIAcube Connect.

Link
Single Cell- Gem beads generation instrument
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10X Genomics |Single Cell & Spatial Transcriptomic Platform

The Chromium X Series instrument enables seamless scaling from pilot experiments through million-cell studies, and offers compatibility with an expanding range of Chromium Single Cell applications. 

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CosMX SMI platform
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Nanostring | Single cell Spatial Transcriptomic Platform

CosMx SMI is the first high-plex in situ analysis platform to provide spatial multiomics with formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue samples at cellular and subcellular resolution. 

Link
smaller NovaSeq 6000 system
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illumina | Next Generation Sequencing

The NovaSeq 6000 system performs whole-genome sequencing efficiently and cost-effectively. Its tunable output generates up to 6 Tb and 20B single reads in dual flow cell mode with streamlined workflows. 

Link

Quality Control Equipment

Services Provided

  • Bulk sequencing (4 different library preps including low input RNA)
  • Single Cell (All 10X Genomics libraries, on fresh or fixed cells)
  • Spatial Transcriptomics (single cell CosMx FF and FFPE)
  • Visium Spatial Transcriptomics upon request
  • Quality Control analysis (RNA – DNA - cells)
  • Consultation, Data Analysis, Grant/Manuscript Assistance

Team

Martina Zoccheddu, PhD

  • Director

Miao Yu, PhD

  • Research Manager

Sahiti Sri Manam, PhD

  • Research Associate