Protocols and Useful Links

Key Resources

Current Protocols in Cytometry is available in hard copy in CN4158D.

Current Protocols in Cytometry is a "best practices" collection that distills and organizes the techniques of peer-reviewed protocols for flow and image cytometry available.

Current Protocols in Immunology is available in hard copy in CN4158D.

Current Protocols in Immunology is also a "best-practices" collection that provides comprehensive coverage of immunological methods.

Purdue Cytometry ListServ

A valuable forum for the discussion of many aspects of flow cytometry is the Purdue Cytometry Discussion List, which has an international following.

Persons may search the discussion archive. To subscribe to the discussion, visit this website. To post an email to the group, send an email to cytometry@lists.purdue.edu.

Links of interest

Reference Links

Commonly Used Staining Protocols - Janis V. Giorgi Flow Cytometry Core Laboratory
BD Biosciences Flow Cytometry Training and E-Learning
ThermoFisher Flow Cytometry Resource Center
BioLegend Web Tools
DNA Analysis Protocols
Cell Cycle Basics
Fluorescence Tutorials -ThermoFisher
G-force calculator for your centrifuge - it's the g-force that's important, not the RPMs!!

Fluorescence Spectrum Viewers

Antibody Cross-Reactivity Resources

Vendor Links

Cytek Biosciences (flow cytometers)
Agilent (flow cytometers)
Thermo Fisher (Attune flow cytometers, Bigfoot sorter (PROPEL Labs), antibodies, etc. - Invitrogen/Molecular Probes/eBioscience/Qdots)
Beckman Coulter (flow cytometers, antibodies, etc.)
Bio-Rad flow cytometry reagents
BioLegend (antibodies, reagents, etc.)
Jackson ImmunoResearch Laboratories (quality secondary antibodies)

Paid Software Links

FlowJo Flow Cytometry Software
FlowJo Tutorials (also check out Flowjo videos on YouTube)
De Novo FCS Express Flow Cytometry Software
Astrolabe Cytometry Platform (cloud based single-cell data analysis)

Free Software Links

MFI Flow Cytometry Software - Eric Martz
Flowing Software - Turku Center for Biotechnology
Bioconductor - an open source software (not specifically for flow cytometry) that uses the R statistical programming language and has some flow cytometry modules

Contact Us

Flow Cytometry

Health Sciences Campus

Georgia Cancer Center - M. Bert Storey Research Building

Jiji Xie, PhD

CN-3142

(706) 721-4157

Rebekah Tritz, PhD
Research Manager

CN-4158E

(706) 721-5468

rtritz@augusta.edu

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