Mass Cytometry

Mass cytometry, or CyTOF (cytometry by time-of-flight), is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes. The readout is performed using time-of-flight mass spectrometry, enabling the simultaneous detection of many more labels in a single sample, without significant channel spillover.

• Simple mix and match of >50 markers
• Single-cell suspension input
• .fcs (FCS 3.0) and .txt data output
• No autofluorescence; no single stained controls and signal compensation or deconvolution required after data collection
• Sample multiplexing/barcoding
• Samples and antibody cocktails are freezable

 

Equipment & Reagent Rates

 

Staff-assisted sample acquisition: $100/hr

Core Antibodies: $7/ul; 

Human MDIPA kit: $270/unit (30-Ab tube)

Technical assistance: $75/hr

 

For more information please go to the Flow and Mass section on the Shared Resources page.