Nuclear Magnetic Resonance
The Nuclear Magnetic Resonance (NMR) instruments can provide structure or molecular
interaction analysis of small organic molecules, peptides, 3D structure of proteins
up to 12-15 kDa, ligand binding to larger proteins, lipids, carbohydrates, nucleic
acids of 8 kDa or less, and selected metals.
The 500 MHz NMR instrument has different probes allowing multiple configurations while
the 300 MHz NMR is limited to proton, carbon, and fluorine studies.
Trained users have 24h access.
Consultation is available to determine recommended experiments, to operate the instrument,
or to assist with data interpretation. Long experiments are frequently restricted
to nights and weekends. Experiments that require a hardware change must be scheduled
in advance.
Default hardware includes a Prodigy Cryoprobe (Inverse probe) for high sensitivity
proton, carbon, nitrogen, and fluorine studies of samples in liquid solution. Available
hardware includes a High Resolution Magic Angle Spinning probe for lipids, cells,
polymers, or high viscosity samples and a Broad Band (Direct probe) for a wide range
of nuclei.
Contact facility to arrange analysis of external or commercial samples. Training is
available for those who wish to become independent users.
- Protein folding
- Molecular dynamics
- Metabolite analysis
- Drug screening and design
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- Ionization state
- Protein hydration
- Chemical analysis
- Native membrane protein
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- Solution structure
- Hydrogen bonding
- Material science
- Weak intermolecular interactions
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Instrumentation
| Field |
300 |
500 |
| Console |
Bruker Avance |
Bruker Avance III |
| RF Channels |
3 |
3 |
| Standard Probe |
Quattro Nucleus Probe (QNP) |
Broad Band probe (BBP) |
| Other Probes, Hardware |
|
Triple Resonance Cryoprobe (H1, C13, N15, F19), high resolution magic angle spinning
probe, 24-tube autosampler |
triangle-exclamation icon General Safety Information
Person with electronic, electrical or mechanical medical implants (such as cardiac
implants, pacemakers, aneurysm clips, surgical clips or prostheses…) MUST remain outside the NMR laboratories and check with the director before entering the
NMR room.
- Do NOT bring any metallic object within 10 feet of any magnet.
- Do NOT bring compressed gas cylinders into the NMR laboratories without NRM personnel supervision.
- All magnetic objects should be kept outside the NMR magnet room. This includes keys,
tools, or mechanical watch, etc. ASSUME any piece of METAL is magnetic until proven otherwise.
- Keep electronics, credit cards and magnetic storage media outside of the NMR magnet
room.
- NEVER put any object into the magnet, except the NRM tube and holder.
- In case of a metallic object strikes the magnet, get facility staff IMMEDIATELY. Do NOT attempt to pull the object off yourself.
- In the event of a magnet quench (sudden and rapid boil-off the magnets cryogens (helium
and nitrogen) that can be detected by visible (and/or audible) emission of cryogenic
gas from the magnet), the magnet cryogens that vaporizes displace air and can result
in asphyxiation. If a quench occurs, IMMEDIATELY EVACUATE THE NMR ROOM!
- For low-temperature NMR experiments, use the non-magnetic nitrogen dewar that is available.
Do not use a wrench to disconnect the quick connector on the dewar.
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Policies
- All users must follow the general rules of the Chemical and Biomolecular Analysis Facility.
- No sample preparation in the NMR lab.
- Do not leave your NMR tubes in the NMR spectrometer.
- If a sample breaks in the magnet, contact the director immediately so the probe can
be cleaned.
- All users must reserve the spectrometer using the iLab.
- All users must sign the spectrometer log-book.
- Non-standard experiments/probe change must be scheduled with the director in advance.
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Research Groups
Siva Panda
- Assistant Professor of Chemistry
Shaobin Miao
- Associate Professor of Chemistry